Coreceptor Tropism and Maraviroc Sensitivity of Clonally Derived Ethiopian HIV-1C Strains Using an in-house Phenotypic Assay and Commonly Used Genotypic Methods
-
Published:2018-08-15
Issue:2
Volume:16
Page:113-120
-
ISSN:1570-162X
-
Container-title:Current HIV Research
-
language:en
-
Short-container-title:CHR
Author:
Kalu Amare Worku1, Telele Nigus Fikrie1, Aralaguppe Shambhu G1, Gebre-Selassie Solomon2, Fekade Daniel3, Marrone Gaetano4, Sonnerborg Anders1
Affiliation:
1. Division of Clinical Microbiology, Department of Laboratory Medicine, Karolinska Institute, Stockholm, Sweden 2. Department of Microbiology, Immunology and Parasitology, Addis Ababa University, Ethiopia 3. Department of Internal Medicine, Addis Ababa University, Ethiopia 4. Division of Infectious Diseases, Department of Medicine Huddinge, Karolinska Institutet, Stockholm, Sweden
Abstract
Objectives:Genotypic Tropism Testing (GTT) tools are generally developed based on HIV-1 subtype B (HIV-1B) and used for HIV-1C as well but with a large discordance of prediction between different methods. We used an established phenotypic assay for comparison with GTT methods and for the determination of in vitro maraviroc sensitivity of pure R5-tropic and dual-tropic HIV-1C.Methods:Plasma was obtained from 58 HIV-1C infected Ethiopians. Envgp120 was cloned into a luciferase tagged NL4-3 plasmid. Phenotypic tropism was determined by in house method and the V3 sequences were analysed by five GTT methods. In vitro maraviroc sensitivity of R5-tropic and dual-tropic isolates were compared in the TZMbl cell-line.Results:The phenotypes were classified as R5 in 92.4% and dual tropic (R5X4) in 7.6% of 79 clones. The concordance between phenotype and genotype ranged from 64.7% to 84.3% depending on the GTT method. Only 46.9% of the R5 phenotypes were predicted as R5 by all GTT tools while R5X4 phenotypes were predicted as X4 by four methods, but not by Raymond’s method. All six tested phenotypic R5 clones, as well as five of six of dual tropic clones, showed a dose response to maraviroc.Conclusion:There is a high discordance between GTT methods, which underestimates the presence of R5 and overestimates X4 strains compared to a phenotypic assay. Currently available GTT algorithms should be further improved for tropism prediction in HIV-1C. Maraviroc has an in vitro activity against most HIV-1C viruses and could be considered as an alternative regimen in individuals infected with CCR5-tropic HIV-1C viruses.
Publisher
Bentham Science Publishers Ltd.
Subject
Virology,Infectious Diseases
Reference67 articles.
1. Berger EA, Doms RW, Fenyo EM. Nature, A new classification for HIV-1.,, 1998, 391,, 240-, 2. Alkhatib G, Combadiere C, Broder CC. Sci, CC CKR5: a RANTES, MIP-1alpha, MIP-1beta receptor as a fusion cofactor for macrophage-tropic HIV-1.,, 1996, 272,, 1955-1958, 3. Feng Y, Broder CC, Kennedy PE, Berger EA. Science, HIV-1 entry cofactor: functional cDNA cloning of a seven-transmembrane, G protein-coupled receptor.,, 1996, 272,, 872-877, 4. Surdo M, Balestra E, Saccomandi P. PLoS One, Inhibition of dual/mixed tropic HIV-1 isolates by CCR5-inhibitors in primary lymphocytes and macrophages.,, 2013, 8,, e68076-, 5. Xiang SH, Pacheco B, Bowder D, Yuan W, Sodroski J. Virology, Characterization of a dual-tropic human immunodeficiency virus (HIV-1) strain derived from the prototypical X4 isolate HXBc2.,, 2013, 438,, 5-13,
|
|