Affiliation:
1. Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China
Abstract
Background:Due to the persistence of latent HIV-infected cellular reservoirs, HIV virus can not be eradicated completely.Objective:To identify proteins related to HIV latency, we performed a subcellular proteomic study in HIV latent cell lines.Method:An established HIV-1 latent cell model (J-Lat Tat-GFP Clone A7 cells, A7 cells) and its parental cell line (Jurkat cells) were used. The plasma membrane (PM) fraction from cultured cells was enriched through aqueous two-phase partition. PM proteins were extracted and then separated using two-dimensional electrophoresis (2DE). Differentially expressed proteins were identified by mass spectrometry, and verified by western blotting.Results:Thirteen non-redundant proteins were identified to be differentially expressed in the A7 PM fraction compared to those in the Jurkat PM. Eight had a PM location through Gene Ontology (GO) analysis. A differential protein network of CAPG-ACTR3-CD3D was detected to have interactions with HIV Vpr, Tat, gp160, etc. through STRING software analysis. One of the differential proteins (Macrophage-capping protein (CAPG)) was verified by western blotting to be down- regulated in two cell lines and HIV resting CD4+ T cells negatively selected from patients.Conclusion:We identified 13 proteins in A7 compared to Jurkat cells. CAPG may be a potential biomarker related to HIV latency.
Funder
Key Issues of Shanghai Municipal Commission of Health and Family Planning and Family
National Natural Science Funds
Publisher
Bentham Science Publishers Ltd.
Subject
Virology,Infectious Diseases
Cited by
6 articles.
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