Analysis of Brassinosteroids in Soybean Seeds and Leaves by Liquid Chromatography-Tandem Mass Spectrometry-Reference-Cited by-同舟云学术

Analysis of Brassinosteroids in Soybean Seeds and Leaves by Liquid Chromatography-Tandem Mass Spectrometry

Published:2017-09-14 Issue:1 Volume:10 Page:100-109
ISSN:1874-2947
Container-title:The Open Plant Science Journal
language:en
Short-container-title:TOPSJ

Author:

Li Hongxia,Trzaskalski Natasha,Neil Emery R.J.

Abstract

Objective:A simple and fast high performance liquid chromatography-electrospray tandem mass spectrometry (LC-ESI-MS/MS) method has been developed for the analysis of brassinosteroids (BRs) in plants without derivatization.Materials:The BRs (including castasterone, 24-epicastasterone, brassinolide and 24-epibrassinolide) have been extracted with ice cold 80% aqueous methanol solution.Method:Five different purification strategies have been tested for the purification and enrichment of BRs.Conclusion:This analytical method was sensitive, reliable, rapid and applicable to trace analysis in complex plant samples.

Publisher

Bentham Science Publishers Ltd.

Link

https://openplantsciencejournal.com/contents/volumes/V10/TOPSJ-10-100/TOPSJ-10-100.pdf

Reference14 articles.

1. Ding J, Mao LJ, Yuan BF, Feng YQ. A selective pretreatment method for determination of endogenous active brassinosteroids in plant tissues: Double layered solid phase extraction combined with boronate affinity polymer monolith microextraction. Plant Methods 2013; 9 : 13-21.

2. Swaczynova J, Novak O, Hauserova E, et al. New techniques for the estimation of naturally occurring brassinosteroids. J Plant Growth Regul 2007; 26 : 1-14.

3. Gamoh K, Takatsuto S. Liquid chromatographic assay of brassinosteroids in plants. J Chromatogr A 1994; 658 : 17-25.

4. Gamoh K, Abe H, Shimada K, et al. Liquid chromatography/mass spectrometry with atmospheric pressure chemical ionization of free brassinosteroids. Rapid Commun Mass Spectrom 1996; 10 : 903-6.

5. Svatos A, Antonchick A, Schneider B. Determination of brassinosteroids in the sub-femtomolar range using dansyl-3-aminophenylboronate derivatization and electrospray mass spectrometry. Rapid Commun Mass Spectrom 2004; 18 (7) : 816-21.

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