Affiliation:
1. Amity Institute of Biotechnology, Amity University Rajasthan, Jaipur 303002, Rajasthan, India
Abstract
Abstract:
The major drawbacks of biofuel production at the commercial level are its low yield, nonavailability
of feedstock, feedback inhibition, presence of inhibitory pathways in various organisms,
and biofuel intolerance of organisms. The present review focuses on the implications of the CRISPRCas9
mediated gene editing tool to alter the genome of bacteria, algae, fungi, and higher plants for efficient
biofuel production. Gene knockout and gene cassette insertions employing CRISPR-Cas9 in
Saccharomyces cerevisiae and Kluyveromyces marxianus have resulted in enhanced production of bioethanol
and 2-Phenyl ethanol in these organisms, respectively. Genomes of several bacterial strains
were also modified to enhance ethanol and butanol production in them. CRISPR-Cas9 modification of
microalgae has demonstrated improved total lipid content, a prerequisite for biofuel production. All
over, CRISPR-Cas9 has emerged as a tool of choice for engineering the genome and metabolic pathways
of organisms for producing industrial biofuel. In plant-based biofuel production, the biosynthetic
pathways of lignin interfere with the satisfactory release of fermentable sugars thus hampering efficient
biofuel production. CRISPR-Cas9 has shown a promising role in reducing lignin content in various
plants including barley, switchgrass, and rice straw.
Publisher
Bentham Science Publishers Ltd.
Subject
Cell Biology,Molecular Biology,Biochemistry,General Medicine
Cited by
8 articles.
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