Affiliation:
1. Department of Pharmacology and Pharmaceutical Sciences, University of Southern California, Los Angeles, CA9033, United States
Abstract
Protein splicing domains, also called inteins, have become a powerful biotechnological
tool for applications involving molecular biology and protein engineering. Early applications of inteins
focused on self-cleaving affinity tags, generation of recombinant polypeptide α-thioesters for the
production of semisynthetic proteins and backbone cyclized polypeptides. The discovery of naturallyoccurring
split-inteins has allowed the development of novel approaches for the selective modification
of proteins both in vitro and in vivo. This review gives a general introduction to protein splicing
with a focus on their role in expanding the applications of intein-based technologies in protein engineering
and chemical biology.
Funder
Crohn's and Colitis Foundation
Congressionally Directed Medical Research Programs
National Institutes of Health
Publisher
Bentham Science Publishers Ltd.
Subject
Cell Biology,Molecular Biology,Biochemistry,General Medicine
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