Affiliation:
1. Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200032, China
2. Key Laboratory
of Theory and Therapy of Muscles and Bones, Ministry of Education, Shanghai, 200032, China
Abstract
Background:
Osteosarcoma is the most common primary bone cancer in children and
adolescents with high metastatic ability.
Aim:
This study aimed to explore the inhibitory effects of (S)-10-hydroxycamptothecin (HCPT)
on osteosarcoma cell growth and metastasis as well as the underlying mechanism.
Methods:
The osteosarcoma cells of 143B and U-2 OS (U-2), treated with HCPT (20, 100, or 300
nM), underwent detections, such as CCK-8, flow cytometry, Transwell, wound healing, and immunoblotting. EMT-related key proteins, like N-cadherin, Snail, and Vimentin, were found to be
down-regulated, while E-cadherin was up-regulated dose-dependently in HCPT-exposed 143B
and U-2 cells. Additionally, incubation of 143B and U-2 cells with HCPT for 3 hours dosedependently reduced the expression ratios of p-LATS1/LATS1, p-MST1/MST1, p-YAP/YAP,
and p-TAZ/TAZ.
Results:
Taken together, our study has demonstrated HCPT to inhibit osteosarcoma growth and
metastasis potentially by activating the HIPPO signaling pathway and reversing EMT.
Conclusion:
HCPT might be a candidate agent for the prevention and treatment of osteosarcoma
Funder
National Nature Science Foundation
National Key R&D Program of China
Shanghai Top Priority Research Center construction project
Shanghai University of Traditional Chinese Medicine
Publisher
Bentham Science Publishers Ltd.