Effects of Oxidative Stress on Serum Metabolism in Acute Pancreatitis Rats Based on Untargeted Metabolomics

Abstract

Objectives: Acute pancreatitis(AP) is a common digestive tract disease, often accompanied by severe metabolic disorders, but there are no specific markers and treatment methods, and the potential metabolic pathways behind it remain to be explored. Methods: Establish mild acute pancreatitis and severe acute pancreatitis models in rats and intervene with antioxidant NAC. Analyze serum oxidative stress indicators and pathological changes in pancreatic tissue. In addition, non-targeted metabolomics analysis of serum differential metabolites between groups was conducted based on the LC/MS system. Results: The pathological score of the model group rats increased, and the levels of oxidative stress factors ROS and MDA significantly increased, while the activity of the antioxidant enzyme SOD decreased. After NAC intervention, oxidative stress damage in rats was alleviated. Non-targeted metabolomics experiments suggest significant differences in serum metabolic profiles among different groups of rats. Conclusion: Metabolomics results show that the obtained differential metabolites are expected to become serum biomarkers for AP. result: In the untargeted metabolomics test, we found that there were obvious differences in serum metabolic profiles of rats. The MAP model group was compared with the NC group, we selected 7 significant differential metabolites, including PC, Tuftsin, etc. The comparison of the MAP + NAC intervention group with the MAP group yielded a total of five significantly differential metabolites, including 20-Deoxynarasin, VPGPR Enterostatin and 12-Ketoporrigenin et al. Compared with the SO group, 23 metabolites with significant differences were screened out in SAP group. The comparison of SAP + NAC intervention group with SAP model group selected 10 significantly different metabolites, including lipids (PC, PE), Physalolactone B, etc.. These metabolites are involved in complex pathophysiological processes such as oxidative stress and inflammation, involving various metabolic pathways such as glycerophospholipid metabolism, arachidonic acid metabolism, and pentose and glucuronate interconversions.