Author:
Ali Syed Abid,Humayun Fozia,Munir Iqra,Ahmad Shakil,Ayub Zarrien,Fatima Habib,Zehra Lakht-e-,Haider Muhammad Samee
Abstract
Objective:
The present study was conducted to investigate the biomass assessment, morphological and molecular identification, nutritive status and biochemical characterization of three major Thais species (T. bufo, T. hippocastanum and T. rudolphi) from the Sindh Coast, Pakistan.
Methods:
Samples were collected from Buleji and Paradise Point at the Sindh Coast. Species were identified morphologically as well as genetically by amplifying two mitochondrial 16S rDNA & Cytochrome Oxidase I (COI) and one nuclear (Histone H3) genes. Shell microstructure and chemistry were also studied by scanning electron microscopy and Energy Dispersive X-ray spectrometry (EDX). The body muscle was dissected and used for nutritional composition determination such as estimation of total protein, carbohydrates, lipids, protein fingerprinting by Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Size-Exclusion - Fast Protein Liquid Chromatography (SEC-FPLC), amino acid and fatty acid analysis.
Results:
Nutritionally, the total protein was found to be the major content followed by carbohydrate and lipid in the three Thais sp. The presence of medicinally important hemocyanin as abundant hemolymph protein was confirmed via SDS-PAGE and SEC FPLC. Nine different types of fatty acids and a high concentration of essential amino acids were also determined.
Conclusion:
Our findings suggest that Thais sp. are nutritionally rich and can be consumed as a valuable marine resource to overcome the malnutrition problem in developing countries.
Publisher
Bentham Science Publishers Ltd.
Reference49 articles.
1. Barkati S, Rahman S.
Species composition and faunal diversity at three sites of Sindh Mangroves.
Pak J Zool
2005;
37
(1)
: 17-31.
2. Afsar N, Siddiqui G, Ayub Z.
Update of records of selected prosobranch gastropod species found along the coasts of sindh and balochistan Pakistan.
Pak J Zool
2012;
44
(1)
: 267-75.
3. Ali SA, Ayub Z, Bano A, Fatima H.
Biochemical studies on siphonaria (gastropoda: pulmonata) from the karachi coast of North Arabian Sea.
Pak J Zool
2011;
43
(6)
: 1085-93.
4. Khan TMA, Razzaq DA, Chaudhry Q-U-ZUZ, Quadir DA, Kabir A, Sarker MA.
Sea level variations and geomorphological changes in the coastal belt of Pakistan.
Mar Geod
2002;
25
(1)
: 159-74.
5. Adeyeye EI.
Waste yield, proximate and mineral composition of three different types of land snails found in Nigeria.
Int J Food Sci Nutr
1996;
47
(2)
: 111-6.
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献