Affiliation:
1. Department of Plastic and Maxillofacial Surgery, Jiangxi Provincial People’s Hospital/Jiangxi Province Key Laboratory
of Maxillofacial Plastic and Reconstruction, Nanchang, China
Abstract
Background:
The healing of cutaneous wounds requires better strategies, which remain
a challenge. Previous reports indicated that the therapeutic function of mesenchymal stem cells is
mediated by exosomes. This work demonstrated the regenerative effects of engineered BMSCsderived
Exosomal miR-542-3p in skin wound mouse models.
Methods:
Bone marrow mesenchymal stem cells (BMSCs) -derived exosomes (BMSCs-Exos) were
isolated by ultracentrifugation and identified by Transmission Electron Microscope (TEM) and
Nanoparticle Tracking Analysis (NTA). BMSCs-Exo was loaded with miRNA-542-3p by
electroporation. We explored the effects of miRNA-542-3p-Exo on the proliferation and migration
of Human Skin Fibroblasts (HSFs)/Human dermal microvascular endothelial cells (HMECs). In
addition, The angiogenesis of HMECs was detected by Tube formation assay in vitro. The effects
of miRNA-542-3p-Exo in the skin wound mouse model were detected by H&E staining, Masson
staining, and immunofluorescence analysis. We assessed the effect of miRNA-542-3p-Exo on
collagen deposition, new blood vessel formation, and wound remodeling in a skin wound mouse
model.
Results:
MiRNA-542-3p-Exos could be internalized by HSFs/HMECs and enhance the
proliferation, migration, and angiogenesis of HSFs/HMECs in vitro and in vivo. The protein
expression of collagen1/3 was significantly increased after miRNA-542-3p-Exo treatment in HSFs.
In addition, the local injection of miRNA-542-3p-Exo promoted cellular proliferation, collagen
deposition, neovascularization, and accelerated wound closure.
Conclusion:
This study suggested that miRNA-542-3p-Exo can stimulate HSFs/HMECs function.
The treatment of miRNA-542-3p-Exo in the skin wound mouse model significantly promotes
wound repair. The therapeutic potential of miRNA-542-3p-Exo may be a future therapeutic strategy
for cutaneous wound healing.
Publisher
Bentham Science Publishers Ltd.
Subject
Immunology and Allergy,Endocrinology, Diabetes and Metabolism
Cited by
4 articles.
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