Comparison of Cytokine Profile between Postmenopausal Women with and
Without Osteoporosis – A Case-Control Study
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Published:2023-05
Issue:6
Volume:23
Page:811-817
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ISSN:1871-5303
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Container-title:Endocrine, Metabolic & Immune Disorders - Drug Targets
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language:en
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Short-container-title:EMIDDT
Author:
Chin Kok-Yong1,
Mohamed Norazlina1,
Soh Geok Ting1,
Mohammad Affaf Hulma1,
Syed Isa Sharifah Nur Liyana1
Affiliation:
1. Department of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Cheras, Kuala Lumpur, Malaysia
Abstract
Background:
Chronic low-grade inflammation is involved in the pathogenesis of postmenopausal osteoporosis, but the cytokines implicated remain elusive.
Objective:
This study aimed to compare the difference in cytokine profile between postmenopausal women with and without osteoporosis in Klang Valley, Malaysia.
Methods:
Postmenopausal women with (n = 20) and without osteoporosis (n = 20) were recruited
for this study. Their bone health status was determined using dual-energy X-ray absorptiometry.
Their fasting blood was collected for proteomic analysis. A protein array was performed for four
subjects randomly selected from each group to screen the potential cytokines. Three cytokines at
least 20% different between groups and consistently expressed by each subject were selected for
validation using enzyme-linked immunosorbent assays (ELISA).
Results:
The protein array screening demonstrated that platelet-derived growth factor-BB, interleukin-
6 receptor (IL-6R), and tissue inhibitor of metallopeptidase-2 were higher in women with
osteoporosis than women without osteoporosis (n = 4 per group), and consistently expressed by
all women. Only body mass index (BMI)-adjusted logarithmically transformed IL-6R levels were
lower among postmenopausal women with osteoporosis compared to women with normal bone
health (p = 0.026) (n = 16 per group) in the ELISA test.
Conclusion:
IL-6R was lower among postmenopausal women with osteoporosis compared to women with normal bone health after adjusting for BMI. However, a large-scale epidemiological study with proteomic analysis needs to confirm the findings.
Publisher
Bentham Science Publishers Ltd.
Subject
Immunology and Allergy,Endocrinology, Diabetes and Metabolism