Análisis bioinformático de las rutas pks y fas en plantas y uso de inductores exógenos sobre la producción de acetogeninas en guanábana cultivada in vitro

Abstract

Goals. To analyze the effect on the production of acetogenins of Annona muricata cultivated in vitro by means of Temporary Immersion Bioreactors (BITs) promoted by the use of exogenous inducers proposed from a bioinformatic analysis. Methodology. A bioinformatic analysis was carried out at the KEGG site of the metabolic pathways of polyketide synthetase (PKS) and fatty acid synthetase (FAS) plants from which acetogenins are derived. Five treatments were performed in the BITs with soursop explants; 1) BAP, 2) BAP + AAs 3) BAP + AAs + Putrescine, 4) BAP + IAA and 5) BAP + IAA + Citrate. BAP and IAA are plant hormones, AAs is a mixture of three ketogenic amino acids, Putrescine and Citrate are inducers of PKS and FAS. Contribution. The cultivation of soursop explants was monitored using BITs of the 5 treatments for 8 weeks without apparent contamination. Sprout observation started from week 6 in treatment 1 and 2 but not in 3 over 8 weeks, with putrescine being more than an inducer a stress factor for the explant as they became necrotic. Treatments 4 and 5 were only monitored until the third week and no information on sprouting was collected. Differences in the profile of acetogenins by HPLC-PDA were observed between soursop leaves to ex vitro and in vitro and between treatments 1, 2 and 3.