Ovum Pick Up (OPU) and in vitro embryo production in pregnant cows (Bos Indicus) at the Bachigualatito rancho, la Trinitaria, Chiapas

Abstract

With the objective of determining the production, quality and development of bovine embryos (Bos Indicus), oocytes were collected from 14 females distributed in two groups: empty females (T1; n=7) and an experimental group of pregnant females (T2; n=7). The two groups were subjected to Ovum Pick Up (OPU) using disposable 18-gauge needles and a vacuum pressure of 100 mmHg. to later carry out in vitro fertilization (IVF). The study variables were the number of oocyte aspirated and fertilized and the expected embryos. A Wilcoxon test was performed for non-parametric data between two groups, finding no significant differences between treatments P> 0.05. 250 oocytes were recovered by fertilizing out of 190, obtaining a fertilization rate of 77%, reaching a transferrable blastocyst rate of 24% (45/190). In empty females (7), 113 oocytes were obtained, reaching a fertilization rate of 75% (81/113) and 25% transferable blastocysts (18/81). In the 7 pregnant females, 137 oocytes were collected, reaching a fertilization rate of 83% (109/137) and 23% transferable blastocysts (27/109). Follicular aspiration for in vitro fertilization of embryos from pregnant and empty cows is viable without differences between them, but being more variable in pregnant cows.