Affiliation:
1. Instituto Tecnológico Superior de Irapuato
2. Universidad de Guadalajara
3. Universidad de Guanajuato
Abstract
Objetive: Analyze the antiparasitic activity of marine invertebrate Ophiocomina nigra in Entamoeba invadens. Methodology. In O. nigra, an analysis of the quantitative proximal chemical composition (moisture, ash, protein, lipids and nitrogen-free extract) was performed. In addition, the determination of the total protein pattern (SDS-PAGE 10%). On the other hand, the antiparasitic activity of E. invadens trophozoites was determined, which were grown in TYI medium at 28 ° C and exposed to the aqueous extract of O. nigra for 24 hours. Subsequently, metabolic activity (XTT assay) was determined and morphology was analyzed. Cytotoxicity tests were performed on human liver cells (Hep G2) exposed for 24 hours to O. nigra (XTT test) and the biomass was determined (violet crystal staining). Contribution. The antiparasitic activity of O. nigra in E. invadens and the cytotoxic effect in human liver cells was determined. There are few scientific studies of this marine invertebrate on its use in traditional medicine, so it is important to analyze its effects and therapeutic value.
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