Hypolipidemic activity of Phaseolus vulgaris (Fabaceae) in mice

Abstract

Objective: To evaluate the lipid-lowering activity of an aqueous extract obtained from the seeds of Phaseolus vulgaris in male mice using the hyperlipidemia induction model with Triton X-100. Methods: the chemical quality of the extract obtained was characterized by quantifying the total polyphenols (Folin Ciocalteu method) and total anthocyanins (colorimetric method) as well as their antioxidant activity by their ferric ion reducing capacity (FRAP, TPTZ method). Results: The data obtained show that the aqueous extract contains a large amount of total polyphenols (415 mg EAG / 100g of seed) and total anthocyanins (43EMG / 100 g of seed) and significant antioxidant activity (11.080.83 of Fex / g of seed). Administration of the aqueous extract to hyperlipidemic mice improved their lipid profile, especially by reducing the serum value of total cholesterol (144 mg/dL) and triglycerides (147 mg/dL) and increasing HDL values (67 mg/dL) in the group that received a dose of 300 mg of extract / kg of weight. Conclusion: These results show that the aqueous extract of P. vulgaris exerts an antioxidant activity in vitro and a lipid-lowering effect in mice.