Functional Anatomy of Whitefly Organs Associated with Squash Leaf Curl Virus (Geminiviridae: Begomovirus) Transmission by the B Biotype of Bemisia tabaci (Hemiptera: Aleyrodidae)

Abstract

Abstract The sweetpotato whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), is a sibling species group that transmits Squash leaf curl virus (SLCV) and other geminiviruses (Geminiviridae, genus Begomovirus) in a circulative and persistent manner. Using in situ hybridization, SLCV was localized in the primary salivary glands, the midgut, and the filter chamber of adults of the B biotype in the group. However, no SLCV particles were localized in the accessory salivary glands. The midgut loop was found to reside, fully or partially, in the abdomen or thorax in >8,000 dissections, indicating that it is capable of moving through the petiole, a constriction between the two body sections. When extended to its anterior-most position in the thorax, the midgut can make direct contact with the salivary glands, but evidence for direct transfer of virions is lacking. However, the widely presumed pathway of viral transport from the gut to the whitefly primary salivary glands can now be broadened to include both the blood and the possibility of direct transfer during contiguity of these two organs. Light microscopical observations indicated that the primary salivary gland consists of a central region flanked by two dark-staining regions, referred to as endcaps. Electron microscopical examination of extirpated and nonextirpated primary salivary glands revealed additional distinct regions and cell types. One such region, located between the central region and an endcap, was correlated directly to the region where virions have previously been immunolocalized.