Analysis of 20-OH-Ecdysone Titers in Ostrinia furnacalis Affected by Azadirachtin Using Capillary Zone Electrophoresis-Reference-Cited by-同舟云学术

Analysis of 20-OH-Ecdysone Titers in Ostrinia furnacalis Affected by Azadirachtin Using Capillary Zone Electrophoresis

Published:2012-11-01 Issue:6 Volume:105 Page:890-895
ISSN:1938-2901
Container-title:Annals of the Entomological Society of America
language:en
Short-container-title:

Author:

Huang Zhiwei¹,Zhao Min¹,Zhou Li²,Shi Ping²

Affiliation:

1. College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, 2999 North Renmin Road, Shanghai, 201620, P.R. China

2. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, P.R. China

Abstract

Abstract A novel technique of insect ecdysone analysis was established by capillary zone electrophoresis using 20-hydroxy ecdysone (purity ≥93%) as a standard sample. It showed that 20-hydroxy ecdysone and other trace impurities were completely separated within 5 min with an electrolyte containing 10 mmol/L borate, at pH 9.18, 20 kV applied voltage and 0.5 psi × 5 s injected volume. Under this optimal condition, effects of azadirachtin on ecdysone in the hemolymph of Ostrinia furnacalis (Guenee) were investigated. The results showed that the peak values of 20-hydroxy ecdysone were strongly suppressed by azadirachtin and the titers remained at a lower level than those in the control samples. When compared with high-performance liquid chromatography, the primary advantages of capillary zone electrophoresis include a much shorter analysis time and detection limit and the elimination of complex pretreatment of samples.

Publisher

Oxford University Press (OUP)

Subject

Insect Science

Link

http://academic.oup.com/aesa/article-pdf/105/6/890/40332925/aesame0890.pdf

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