High Viral Load in the Planthopper Vector Delphacodes kuscheli (Hemiptera: Delphacidae) is Associated With Successful Transmission of Mal de Río Cuarto Virus

Author:

Caro Evangelina B Argüello1,Maroniche Guillermo A2,Dumón Analía D1,Sagadín Mónica B1,Vas Mariana Del2,Truol Graciela1

Affiliation:

1. Instituto de Patología Vegetal-Centro de Investigaciones Agropecuarias-lnstituto Nacional de Tecnología Agropecuaria (IPAVECIAP-INTA). Av. 11 de Septiembre 4755 B° Cárcano. Ciudad de Córdoba CP X5020ICA, Córdoba, Argentina

2. Instituto de Biotecnología-Centro de Investigación en Ciencias Veterinarias y Agronómicas-lnstituto Nacional de Tecnología Agropecuaria (IB-CICVyA-INTA). Las Cabanas y Los Reseros s/n. Hurlingham CP 1686, Buenos Aires, Argentina

Abstract

Abstract Delphacodes kuscheli Fennah (Hemiptera: Delphacidae) is the main natural vector of Mal de Rio Cuarto virus (family Reoviridae, genus Fijivirus, MRCV), which infects different gramineae and causes the most important maize (Zea mays L.) disease in Argentina. MRCV—vector interactions usually are studied using different winter cereals as hosts. Under experimental conditions, <50% of D. kuscheli planthoppers fed on a MRCV-infected plant can transmit the virus to wheat (Triticum aestivum L. ). This fact is influenced by insect development stage at acquisition and the latency period. This work describes the relation between transmission efficiency and MRCV accumulation in its planthopper vector. First- and third-instar D. kuscheli nymphs were allowed to feed on MRCV-infected plants, and 9 or 17 d after the acquisition access period (AAP), viral load of transmitting and nontransmitting planthoppers was quantified by quantitative polymerase chain reaction. The transmitting planthoppers showed significantly higher viral titers than nontransmitting ones, suggesting that successful transmission is positively associated to viral accumulation in the insect. However, planthoppers of the third-instars group did not transmit the virus 9 d after AAP, even when 46% had similar titers to the transmitting insects of the other treatments. These results indicate that additional factors influence MRCV transmission efficiency when acquisition occurs in older planthoppers. This is the first precise quantitative analysis of MRCV in its main vector species and will definitely contribute to better understand planthopper—Fijivirus interactions and its epidemiological implications.

Publisher

Oxford University Press (OUP)

Subject

Insect Science

Reference34 articles.

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3. Transmisión del Mal de río cuarto virus por ninfas de primer y tercer estadio de Delphacodes kuscheli.;Arneodo;Pesqui. Agropecu. Bras.,2005

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