CELLULOSE DESTRUCTIVE ACTIVITY OF SOIL MICROFLORA AT THE INFLUENCE OF DIFFERENT RADIONUCLIDE CONTAMINATION LEVELS

Author:

Illienko V. V.,Volkogon I. V.,Bordyug O. A.,Klepko A. V.,Lazarev M. M.,Gudkov I. M.

Abstract

The results on impact of different levels of soil radionuclide contamination on microbiota and cellulosedestroying microflora obtained. The state of this microflora is one of the indicators of the overall soil microbial activity. In the remote period after Chernobyl accident, there is a problem of the agricultural use of radionuclide contaminated territories and returning to economic activity the lands that withdrawn from circulation in 1991-1996. We used the following research methods - spectrometric, radiochemical analysis, microbiological, agrochemical and statistical analysis. For field research in the zone of unconditional (compulsory) resettlement and exclusion zone of the Chernobyl NPP, two sites selected and inspected, within which the burial places for plant material identified and soil samples took for radiological and agrochemical analysis. For sites, a significant gradient in radiological parameters observed. The radiation background varies from 0.13±0.01 to 34.8±0.5 μSv/h. The radionuclides activity concentrations in the soil of sites is characterized by the following parameters: 137Cs - from 600±45 to 203800±4100 Bq/kg, 90Sr - from 33±4 to up to 34000±300 Bq/kg. The absorbed dose rate for microbiota showed maximum values up to 1.57 μGy/h at the 1st site and up to 84.00 μGy/h at the 2nd site. Data on cellulosolytic activity of soil microorganisms of both sites were obtained, TВI index coefficients were calculated and the number of soil microorganisms was estimated based on the results of sowing soil suspension on nutrient media.

Publisher

National University of Life and Environmental Sciences of Ukraine

Subject

General Medicine

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