Analysis of methods of diagnosis of bacterial diseases of tomatoes in Ukraine

Abstract

Effective and economical methods of detection and identification necessary for the study of ecology, pathogenesis and limiting the spread of phytopathogenic bacteria, as well as for seed certification programs. The available methods for the detection and identification of four main causative agents of bacterial diseases of tomatoes Xanthomonas vesicatonia, Clavibacter michiganensis subsp. michiganensis, Pseudomonas syringae pv. tomato, Pectobacterium carotovorum subsp. carotovorum, which are common in Ukraine. These methods include microbiological using selective media, serological, molecular methods based on the amplification of unique DNA sequences. The use of modern biochemical test systems has eliminated the need to identify phytopathogenic bacteria using long-term routine methods. Commercially available commercial test systems provide rapid identification at the species level and also detect new and quarantine pathogens. PCR-based methods have advantages over traditional diagnostic tests because isolates do not need to be cultured before detection and protocols are highly sensitive and fast.