EXPOSURE TO DISINFECTANTS OF VARIOUS CHEMICAL NATURE ON THE CULTURE OF PATHOGENIC LEPTOSPIRA

Abstract

Infectious diseases cause substantial economic damage to livestock farms, so there is a constant search for new means of preventing diseases, especially disinfectants. Analysis of the scientific literature indicates a substantial problem of leptospirosis in Ukraine and there are virtually no data on the use of complex oxidising drugs for its prevention. The purpose of the work was to examine the effect of Biolide (active substances hydrogen peroxide, lactic and supralactic acids) and Diolide disinfectants (active substances sodium chlorite and sodium chloride) on the causative agents of leptospirosis. The stability of eight pathogenic Leptospira cultures of different ages circulating in Ukraine and their growth properties were tested by adding different concentrations of these disinfectants to them. The results obtained were statistically analysed in the Epitools – Epidemiological Calculators software. Effective concentrations and exposures of Biolide and Diolide for use in preventive and forced disinfection in leptospirosis were determined. As a result of studies on the effect of both disinfectants on 7-, 10- and 15-days Leptospira test cultures, no differences were recorded between the indicators of their accumulation (number of microbial cells/cm3). Therefore, the results obtained for cultures of different ages were considered as repeatability. It is proved that for preventive and forced disinfection in leptospirosis, a 0.55% solution of Biolide is recommended for use at an exposure of 30 minutes at a temperature of 24°C. If the exposure period is increased to 60 minutes, it is allowed to reduce the concentration of the product to 0.185%. Regarding the drug “Diolide”, it is recommended to use it in this zoonosis in a dilution of 200 mg/l (concentration of 0.08% of the active substance) during exposure for 15 minutes at a temperature of 24°C. If the exposure period is increased to 30 minutes, it is allowed to reduce the dilution of the drug to 50 mg/dm3 (concentration of 0.02% of the active substance). In addition, it was determined that both disinfectants completely inhibit the growth of pathogenic cultures of Leptospira. The practical value of the study is to prove the possibility of using complex disinfectants based on oxidising agents for the prevention of leptospirosis