Author:
Liasota Vasyl,Bohatko Nadiia,Tkachuk Svitlana,Bukalova Nataliia,Khitska Oksana
Abstract
The relevance of this study is conditioned upon the need to improve the hygiene of milking cows by selecting detergents and disinfectants with different component compositions that ensure the quality and safety of dairy products. The purpose of this paper was to investigate the effectiveness of solutions of modern chlorine-containing and chlorine- free detergents and disinfectants on the test cultures of microorganisms, the sanitary and hygienic condition of the milking parlour, parts of milking equipment, and the skin of the udder teats of cows. The total number of bacteria was determined according to the requirements of DSTU ISO 15214:2007, by inoculating 1 cm3 of the test material on meat-peptone agar followed by incubation at an average temperature of 36°C for 24-48 h. Cultivation was performed in a thermostat at 37°C. After incubation, colonies of grown microorganisms were counted, and the number of colony-forming units was determined. Detergents and disinfectants “Chloramine B”, “Sanalcalin” and “Neochlor” have a detrimental effect on the growth of test cultures of E. coli, S. aureus and P. aeruginosa. “Desmol” suppresses the growth of test cultures of E. coli and P. aeruginosa after 20 minutes. The number of colonies of microorganisms in the air of the milking parlour is minimal at the beginning and greatest at the end of milking. 0.5% solution has a detrimental effect on E. coli and S. aureus, and slightly affects the growth of Salmonella spp. At the same time, this product is effective against microbial contamination of the skin of the udder teats of cows for its use in pre-milking disinfection. During the treatment of milking equipment with a 0.5% “Gralan Gel” solution, the total bacterial inoculation of the milking gum is reduced by 94.1%, the collector – by 98.4%, the milk hose – by 96.5%, the tank of the milking machine – by 97.4% compared to the indicators before processing the milking equipment with detergents and disinfectants. According to the results of the study, the best efficiency in reducing the general bacterial contamination of milking equipment, the harmful effect on opportunistic microflora and microbial contamination of the skin of the udders of cows when used in pre-milking disinfection at milk production enterprises shows a 0.5% solution of the alkaline detergent and disinfectant “Gralan Gel”. Thus, it is advisable to use a 0.5% solution of alkaline detergent and disinfectant “Gralan Gel” for producers of cow’s raw milk to reduce the total bacterial contamination of milking equipment and cow udders
Publisher
National University of Life and Environmental Sciences of Ukraine