CYTOLOGICAL CHARACTERISTICS OF EXPERIMENTAL PURULENT NON-HEALING WOUNDS IN THE TREATMENT OF SILVER NANOPARTICLES

Abstract

Summary. The purpose of the work is to conduct a cytological examination of swabs-imprints of experimental purulent wounds that have not healed for a long time, when treated with silver nanoparticles in comparison with 0.05 % Chlorhexidine solution. Research materials and methods. The study of the features of the cytological picture of skin regeneration was carried out on 60 white non-linear laboratory rats. Research results. The obtained results of the cytological examination of purulent wounds that did not heal for a long time indicate faster wound healing processes in the group using nanostructured silver compared to the control group: there was a faster decrease in the number of leukocytes in the field of view and the percentage of their destruction, faster activation of monocyte-macrophage cells series, the appearance of fibroblasts and epithelial cells and, as a result, a faster change in the types of cytograms from necrotic to regenerative. Conclusions. It was established that the presented method of using nanostructured silver contributes to a better elimination of microbial insemination, leads to a significant decrease in the number of neutrophil leukocytes and leukocytes in the stage of destruction, an increase in the percentage of phagocytic leukocytes, fibrocytes, fibroblasts, endotheliocytes and epithelial cells, which indicates a better antibacterial and anti-inflammatory effect and indicates much faster processes of cleaning and regeneration of purulent wounds. The use of silver nanoparticles has significant advantages compared to traditional treatment with a 0.05 % solution of Chlorhexidine and is promising for use in the practice of purulent surgery.