THE USE OF SUTURE MATERIAL WITH ANTIOXIDANT PROPERTIES FOR INTESTINALORRHAPHY UNDER THE CONDITIONS OF ACUTE PERITONITIS I N AN EXPERIMENT

Abstract

Summary. Aim. Study of the effectiveness of the use of suture material with antioxidant properties for the prevention of suture failure in the small intestine (SmlI) under conditions of acute peritonitis (AP). Materials and methods. The research material was 70 white rats. AP was simulated in 63 animals. After 12 hours, a laparotomy was performed. In 56 animals, after washing the abdominal cavity, the SmlI was dissected and sutured. Kapron was used in the control (C), in experiment (E) - kapron impregnated with a 5 % solution of ethyl methylhydroxypyridine succinate. In 7 intact rats, 7 with AP models, and 1, 3, 5, and 7 days after SmlI suturing, sections of SmlI were taken for histological examination. The preparations were stained with hematoxylin-eosin, according to Mikel Calvo, the R/B ratio (R/BС) was determined. Results and their discussion. 12 hours after AP modeling, the R/BC indicators in the connective tissue fibers of the submucosal base of the TC increased significantly (p<0.05). 1 day after suturing, R/BC indicators increased significantly (p<0.05) in both C and E. After 3 days, R/BC indicators increased in C and significantly decreased in E (p<0.05) . In E, fibrin was found between the fibers of the ligatures. After 5 days, the indicators of R/BC in C and E significantly decreased (p<0.05), but in E they were significantly lower (p<0.01). In C, fibrin was found between the fibers of the ligatures, in E — granulation tissue. After 7 days, R/BC indicators in C and E decreased, but in E they were significantly lower (p<0.01). In C, unfilled areas remained in the granulation tissue between the fibers of the ligatures. Therefore, after suturing the wound of the small intestine in animals with AP models with kapron ligatures impregnated with a 5 % solution of ethyl methylhydroxypyridine succinate, according to histological studies, the reduction of the oxidation activity of the proteins of the intestinal tissues adjacent to the ligatures and the regeneration of the areas of the sutures are accelerated. Conclusions. 1. 12 hours after the simulation of acute peritonitis in rats, the R/B ratio increases statistically significantly (p<0.05) in the connective tissue fibers of the submucosal base of the small intestine. 2. 1 day after suturing the wound of the small intestine, regardless of the type of ligatures used, in animals with models of acute peritonitis, the indicators of the R/B ratio increase statistically significantly (р<0.05). 3. In the future, within 7 days, after using ligatures impregnated with a 5 % solution of ethyl methylhydroxypyridine succinate, the R/B coefficient indicators are statistically significantly reduced and remain statistically significantly lower than the control indicators, at the same time, there are signs of relatively accelerated tissue regeneration in the areas of applied sutures.