PHOTODYNAMIC THERAPY OF PURULENT WOUNDS IN EXPERIMENTAL DIABETES MELLITUS

Abstract

The aim of the study was to study the effectiveness of the method of photodynamic therapy of purulent wounds in conditions of experimental diabetes mellitus. Materials and methods. Experimental studies were carried out on 30 mature male Wistar rats, which were injected with diabetogenic cytotoxin streptozotocin to induce type 2 diabetes mellitus, and also simulated a purulent wound. Various combinations of topical treatments have been used for treatment. A comparative analysis of the use of various methods of treating purulent wounds was carried out using the following methods: clinical, bacteriological, planimetric, histological, and statistical data processing. Results and discussion. In the course of clinical observation, it was found that a day after the modeling of infected wounds, the general condition of the animals could be assessed as moderate: the animals were lethargic, inactive, some of them had sanious discharge from the nose. Wounds in all animals had signs of suppuration: accumulation of liquid fibrinous-hemorrhagic exudate was noted in the cavity of the ring, in some animals there was no exudate. in two experimental groups (B and C), PDT sessions were performed according to the plan; in the control group (A), the wounds were treated with chlorhexidine solution. During the experiment, it was shown that the proposed method for the treatment of purulent wounds against the background of diabetes mellitus makes it possible to optimize the wound process, reduce the time for the appearance of granulations and the filling of mature granulation wounds. The use of photodynamic therapy contributed to the rapid cleansing of wounds, the appearance and marginal epithelization. Microbiological examination testified to the rapid decontamination of wounds. Сonclusions. The use of the photosensitizer 5-ALA in the complex of wound treatment under conditions of photoactivation enhances the antibacterial, anti-inflammatory and pro-regenerative effects; This opens up the possibility of a promising use of this complex for antibacterial photodynamic therapy as a new method for the treatment of infectious and inflammatory diseases of the skin and soft tissues in diabetes mellitus.