A cross sectional study optimizing platelet rich plasma preparation using various centrifugation speeds and its effects on the platelet yield

Abstract

Platelet-rich plasma (PRP) is an autologous preparation of platelets in concentrated plasma (with usually >1,000,000 platelets/μL or 2–7 times the concentration in whole blood), prepared by a process known as differential centrifugation. The therapeutic efficacy of a PRP preparation is largely determined by its platelet concentration. Although PRP has been used for many years, there is no standard preparation protocol. This study was conducted to compare different protocols to determine which spin variation produces the highest platelet yield.A cross sectional study was conducted at a tertiary care hospital with 35 patients. We included the data of the patients that were enrolled in PRP therapy for various dermatological indications. We analysed the data for PRP prepared using different centrifugation protocols and then compared the platelet count of PRP with the baseline platelet count of whole blood to obtain the platelet yield. The platelet yields for different protocols were compared using statistical analysis (IBM SPSS software).After comparing the platelet yield of different protocols, we observed that centrifugation at 1300rpm/246 g for 20 minutes followed by 1600 rpm/373 g for 20 minutes achieved the highest platelet yield-4.6468 times as compared to the mean platelet count in the whole blood and the difference was statistically significant.Multiple parameters influence the platelet concentration obtained in PRP. Therefore, the practitioner must consider the method of PRP preparation that gives the optimum platelet yield to deliver the best therapeutic results to the patients.