Antitussive effect of granule formulation on SO gas-induced cough in experimental animals

Abstract

All over world cough is a common symptom in respiratory disease. When cough becomes severe, opioids act as a potent drug, but they have various side effects like sedation, constipation, etc. Therefore, there is a need to have an effective antitussive formulation, which doesn’t have respiratory depressant activity. The present study was carried out to evaluate the antitussive activity of granules containing extract using a cough model induced by Sulphur dioxide gas in experimental mice. The antitussive effect of granule formulation on SO gas induced cough in experimental animals, which compared to standard codeine sulphate and the result was determined by statistical analysis. The antitussive activity of the granules tested in control, standard and test animal group respectively, it was compared to standard codeine sulphate (10, 15, 20 mg/kg body weight). Codeine sulphate as a standard drug for suppression of cough, act as potent antitussive agent, which produced 25.29%, 33.33%, 47.13% inhibition in cough at a dose of 10mg/kg, 15mg/kg and 20mg/kg respectively, whereas, codeine sulphate (20mg/kg) showed a maximum inhibition of 47.13% (p<0.01) after 60 min. of experiment. The test group of mice was showed 41.17%, inhibition, in cough on treatment with granules after 60 min. of an experiment. This is very significant or nearly equal to a maximum dose of codeine sulphate (20mg/kg). Statistical analysis shows very significant antitussive effects of granules at the level of p<0.01 in inhibiting the cough reflex at 200 mg/kg body wt. in comparison to the control group.