Coenzyme Q10 and N-acetyl cysteine modulates the haematological parameters, markers of oxidative stress and membrane bound phosphatase in spleen toxicity induced by aniline hydrochloride

Abstract

Aniline is widely used chemical for industrial purpose. Exposure to aniline can lead to various health issues in human being. The most common toxic effect of aniline is splenotoxicity which is produced via the generation of oxidative stress. The present study examines the protective effect of coenzyme Q10 and N-acetyl cysteine on spleen weight, body weight, endogenous antioxidants and membrane bound ATPases in aniline hydrochloride (AH) induced spleen toxicity in rats. Adult male albino rats were divided into five groups, each group consists of six rats. Toxicity was induced by administration of AH (100 ppm, p. o) in drinking water for 30 days. Body weight, markers of oxidative stress and haematological parameters were assessed at the end of treatment period. Treatment rats received Coenzyme Q10 (CoQ10, 10 mg/kg/day/p.o) and N- acetyl cysteine (NAC, 300mg/kg/ day p.o) alone and in combination for 30. AH toxic rats showed a significant elevation of spleen weight, WBC count, iron content, LPO level, NO level and Ca ATPase whereas a significant decrease in body weight, haemoglobin, RBCs level, Protein content, GSH and Na/ K and Mg ATPase were observed. Administration of Coenzyme Q10 (CoQ10, 10 mg/kg/day/p.o) and N- acetyl cysteine (NAC, 300mg/kg/ day p.o) together for 30 consecutive days shows a significant decrease in spleen weight, WBC level, iron content, LPO level, NO level whereas significant increase in body weight, hemoglobin level, RBCs level Protein content, GSH level, Na/ K ATPase, Ca and Mg ATPase when compared with aniline treated group and CoQ10 or NAC alone treated groups. These findings indicate the synergistic & protective effect of CoQ10 and NAC in aniline induced spleen toxicity in rats compared to alone antioxidants.