Estimation of eugenol in different tulsi ayurvedic formulations by RP-HPLC method

Abstract

The aim of the present is to develop simple, accurate and precise RP-HPLC method for the quantification of Eugenol content in various tulsi based ayurvedic formulations. HPLC was carried out by reverse –phase technique on a C18 column with a mobile phase composed of Acetonitrile: Methanol in the proportion of 50:50 v/v, at a flow rate of 1.0 mL /min. The detection was carried at 281 nm by UV- Visible detector. The linearity was established in the concentration range of 10-50 mcg/mL with an R value of >0.999. Method and system precision was established with an RSD of < 2%. Accuracy was performed with standard addition spiking method and the results were found to be good. Robustness was established for change in flow rate, mobile phase composition and wavelength of detection. The assay of eugenol was performed in Eugenol drops, tablets, syrup and mother tincture and the results were reported. The method was found to be specific, precise, accurate and robust. The proposed method can be used for the reliable quantification of Eugenol.