Protective role of hydroxy citric acid (HCA) against lead induced toxicity in albino wistar rats

Abstract

The present study was designed to evaluate the role of herbal active constituent hydroxycitric acid obtained from Garcinia cambogia and Hibiscus subdariffa in heavy metal especially lead poisoning. The Lead acetate at 500ppm was used as an inducing agent in the present study. Sodium acetate (500ppm) used as control group is used as a baseline measure. The HCA at 100 mg/kg and 200mg/kg were used in the treatment of lead induced toxicity in rats. At the end of 28 days study period the blood levels of alpha ALAD activity was estimated in all the treatment groups. Lead inhibits the ALAD is more profound and its inhibition has been used clinically to gauge the degree of lead poisoning. Inhibition of ALAD results in the accumulation of aminolaevulinic acid, detectable in the plasma and urine even at blood lead levels of less than 10 µg/dl. It results that HCA showed significant elevated levels of ALAD activity, which was reduced by the lead intoxication. HCA showed significant reduction of liver enzymes (SGOT, SGPT and ALP). The markers like protein, bilirubin and creatinine were also found to be elevated in lead intoxicated rats and was found to be decreased significantly in a concentration dependent manner.