Colorimetric variations in cadaveric tissues embalmed with modified Thiel\\\'s and Formalin solutions: An objective comparative study using image J

Abstract

The comparison of tissue colour between Thiel's embalmed cadavers (TEC) and formalin-embalmed cadavers (FEC) is crucial for assessing the efficacy of Thiel embalming technique as a suitable alternative to formalin embalming for surgical skill training and anatomical education. Most of the studies which declare the superiority of tissue colour in TEC over FEC have done subjective assessment and comparison of the tissue colour. This study aims to compare the colour difference between Thiel and formalin embalmed cadavers objectively. : The current study used six human cadavers to compare the effects of two embalming methods, Modified Thiel’s solution (MTS) and Modified Formalin Solution(MFS). The tissues of the cadavers were dissected and photographed to be analysed for Red, Blue, Green values using Image J software.  The mean (SD) R-value (Red), G-value (Green), and B-value (Blue) for MTS embalmed cadavers were 149.78 (16.070), 124 (19.675), and 109.96 (9.852), respectively, from pictures of embalmed cadavers from research groups. The mean RGB value of MTS embalmed cadavers was substantially (P<0.05) higher than MFS embalmed cadavers.  The current research found that MTS-embalmed cadavers have greater RGB values than MFS-embalmed ones. This information helps to fully understand tissue property changes, evaluate their impact on student perceptions and learning outcomes, and determine Thiel embalming's suitability for surgical training and workshops.