Titanium platelet–rich fibrin (T-PRF) as doxycycline delivery system: an in-vitro study

Abstract

: Locally delivered anti-infective agents are one of the frequently used treatment strategies for the treatment of periodontal disease. Doxycycline (Doxy) is frequently preferred in periodontal therapy. Various carrier systems are available like Collagen sponges, mucoadhesive polymers. The use of PRF is a good strategy for drug delivery systems with its 3D matrix structure as a scaffold, as it can naturally degrade within a certain period of time without causing any allergic/inflammatory conditions in the body. Titanium platelet–rich fibrin (T-PRF) has a denser fibrin structure than PRF with long resorption time and may be a good candidate for long-term drug delivery and release.: To study and compare physical and antibacterial properties of T-PRF and Doxycycline loaded T-PRF.: It is an in vitro study. The T-PRF and T-PRF loaded with Doxycycline as T-PRF/Doxy was prepared and their physical properties like length and consistency was evaluated. Antibacterial activities against and will be investigated by measuring zone of inhibition after 24 hrs and 48 hrs.The antimicrobial activity of T-PRF against significantly increases after addition of doxycycline (p<0.05) without significantly altering physical property of T-PRF.: T-PRF loaded with doxycycline can be used as local drug delivery agent. However, very little literature is available in this field. And in vivo studies are required to determine the efficiency in periodontitis and peri-implantitis cases.