Comparative evaluation of antimicrobial efficacy of QMix 2in1, Octenidine Dihydrochloride irrigants, 940nm diode laser with and without NaOCl, against mature E. faecalis biofilm: An in vitro study

Abstract

To compare the antimicrobial efficacy of NaOCl, QMix 2in1, Octenidine dihydrochloride, 940nm diode laser with and without NaOCl against matured E. faecalis biofilm.Ninety extracted single-rooted teeth were instrumented and autoclaved. The specimens were exposed to E. faecalis contamination over a period of 21 days and subsequently divided into six distinct groups through a random assignment process. Group I-Control, Group II-NaOCl, Group III-940nm Diode laser, Group IV-940nm Diode laser + NaOCl, Group V-QMix 2in1, Group VI-Octenidine Dihydrochloride. Microbial specimens were gathered, cultured, and the quantification of colony-forming units was conducted.ANOVA, Post hoc test, And Tuckey test were applied to find significance.940nm Diode laser +NaOCl group showed a greater antimicrobial effect compared to other groups. No significant difference was observed between 940nm Diode laser and 940nm diode laser +NaOCl (p=.550), NaOCl and QMix 2 in 1 (p=.121), NaOCl and 940nm Diode laser groups (p =.680)The concurrent use of both sodium hypochlorite and 940nm Diode laser light manifests a synergistic influence, enhancing the bactericidal efficacy. The antibacterial effect of QMix 2in1 is comparable to that of NaOCl, 940nm Diode laser, more effective than OCT and less effective than the combination of sodium hypochlorite and 940nm Diode laser.A combination of 940nm laser and NaOCl is effective in reducing the bacterial count because of their capacity to penetrate effectively deep into dentinal tubules, resulting in intensified bactericidal action compared to newer irrigants used alone.