Evaluation of efficacy of various remineralizing agents on artificially demineralized human enamel – An in-vitro study

Abstract

Currently much research revolves around remineralization as incidence of white spot lesions in patient undergoing orthodontic treatment is 2-96%. The objective of this study was to find out the efficacy of Casein phosphopeptide-amorphous calcium fluoride phosphate (CPP-ACFP), Functionalized tricalcium phosphate (f-TCP), Calcium sodium phosphosilicate (CSPS), Calcium sucrose phosphate (CSP), Nano hydroxyapatite (nHAP) and Fluoride (F) in remineralizing artificially demineralized enamel and DIAGNOdent and scanning electron microscope were used to record the changes. The study included 140 extracted premolars which were divided into 7 groups in which 6 were experimental groups: I (CPP-ACPF), II (f-TCP), III (CSPS), IV (CSP), V (nHAP), VI (F) and 1 control group: VII (C). All the samples were evaluated using DIAGNOdent at the baseline, post demineralization and post remineralization. One sample was randomly selected from each group for evaluation using SEM. Statistical analysis has shown that all the groups had higher amount of remineralization except control group. CSPS showed maximum remineralization. All the artificially demineralized samples treated with remineralizing agents for 3 weeks reduced the severity of lesions by forming calcific deposits. However CSPS showed marginally more amount of remineralization than others.