Comparative study of extraction and analysis of DNA from different temperatures using phenol chloroform method

Abstract

The extraction of DNA from biological samples is a fundamental process in forensic investigations, providing invaluable information for the identification and profiling of individuals involved in criminal activities. Saliva is a commonly encountered biological fluid at crime scenes and can serve as a rich source of DNA evidence. This article focuses on the extraction of DNA from saliva samples and explores the methods and techniques employed in forensic investigations to optimize DNA recovery and maximize profiling success. Saliva samples were collected in saline in a swab tube and half of the samples were kept at 4C and half at Room Temperature (RT) to understand the temperature variance. After that, DNA extraction was done using the phenol-chloroform method. The quality and quantity of the extracted DNA are assessed using established techniques such as Gel electrophoresis and qPCR amplification. The results of the experiments reveal the efficiency and reliability of the DNA extraction method for saliva samples. DNA was successfully extracted from the set conditions. Although the DNA bands obtained from 4oC were more prominent compared to the samples kept at RT. The identification of optimal DNA extraction methods for saliva samples can contribute to the development of standardized protocols and guidelines for forensic DNA analysis. In conclusion, this article sheds light on the extraction of DNA from saliva samples for its use in forensic investigation. The outcomes of this study have the potential to improve the efficiency and effectiveness of forensic investigations. Further research in this field is warranted to explore emerging technologies and refine existing methodologies for DNA extraction from saliva samples in forensic contexts.