Determination of the Relationship Between the Number of Clusters and Primary Bud Size in Vitis vinifera and Vitis labrusca Grapevines by Histological Sectioning Method

Abstract

This study was conducted to reveal the relationship between the number of clusters and of primary buds size in dormant grapevine winter buds. In addition, it has been tried to determine how the size of the primary bud and the cluster primordials in it change according to the position on one-year-old shoots. In this study, Vitis vinifera L. cv. ‘Karaerik’ and Vitis labrusca L. cv. 'Ülkemiz' varieties was used. Primary buds are separated from other shoot beds (secondary and tertiary buds) with the a scalpel, and fixation, vacuuming, paraffin impregnation, paraffin embedding, freezing, sectioning and tissue staining were performed, respectively. The stained samples were examined microscopically, images were taken and these images were transferred to the computer and histological analyzes was made. In the study, it was determined that bud sizes including three clusters were statistically different (p < 0.05) from other bud sizes (without clusters, one cluster and two clusters of buds). It was determined that there were statistical differences between cultivars in terms of both bud size and the number of cluster. In addition, it was determined that the middle (0.77) buds of the ‘Karaerik’ variety contained more clusters than the basal (0.66) and apical (0.59) buds, whereas the apical (1.19) buds of ‘Ülkemiz’ variety contained more clusters than the basal (0.88) and middle (0.98) buds. It is very important to know the structural features of the buds in order to make the right pruning application suitable for the variety.