Effects of Osmotic Drought and GA3 Applications on Germination, Seedling Growth and Mitotic Index of Rye

Abstract

In this study, effect of gibberellic acid (GA3) and putrescine of polyamines on germination, seedling growth and mitotic index were analyzed on rye seeds cv. Aslım-95 under osmotic drought conditions formed by using polyethylene glycol (PEG) 6000 application. In order to form osmotic drought, test was conducted by combining 6 different doses of PEG as 0, -2, -4, -6, -8 and -10 including the control (0) and the doses of GA3 as 0, 300, 600, 900 mM. The concentration of -2 bar of polyethylene glycol (PEG) 6000 that was conducted so as to form osmotic drought influenced positively some characters that were examined in the study. However, the incentive effect of -2 bar of osmotic drought showed few change in compliance with PEG 6000+GA3 combinations. On the other hand, following that concentration, when osmotic drought level, that is PEG 6000 concentration, became higher, all the characters were influenced in a negative way. All the doses of GA3 that was used in combination with PEG 6000 showed positive effects on the examined characters, and shortened the germination time, that is, germinating duration. The biggest effect was observed in the concentration of 900 mM which is the highest concentration of GA3. As a result, especially high doses of osmotic drought stress affect germination and seedling growth in a very negative way; It was determined that all doses of GA3 could partially compensate for the effect of osmotic drought induced by PEG 6000.