Effect of Sodium Azide Treatment at Different Duration and Concentration on germination and Seedling Growth Characters in Wheat (Triticum aestivum L.)

Abstract

Wheat in the world and in Turkey to be essential nutrients to maintain the leading status in the still, as a result, it is widely cultivated. Therefore, it is important to increase the genetic and physiological diversity in wheat. Mutation breeding is an effective method for increasing genetic diversity in cultivated plants. The aim of this study is the identification of different duration [control (10 min), 1 h, 2 h and 3 h] effects and concentrations [0 (control), 0.5, 1, 1.5 and 2 mM] of sodium azide (NaN3) mutagen in germination and seedling growth on wheat (Kırik cultivar) and determine the optimum doses of sodium azit which can be used in breeding programs. This study was conducted in 4 replicates according to the completely randomized factorial design. Some germination and seedling characters were investigated in this study. The results showed that except the germination percentage application of sodium azide in different concentrations and duration were significantly affected all considering traits. We believe that increase concentration and duration treatment of SA leads to decreases germination rate index and germination vigor index despite that means time to germination also was increased. Likewise, seedling growth characters significantly were affected by NaN3 application concentration and duration. Also based on the value of LD50 2 h treatment + 1 mM concentration was determined for the optimum dose of the applied root and shoot length.