Affiliation:
1. Central Siberian Botanical Garden, Siberian Branch of the Russian Academy of Sciences; Novosibirsk State Agrarian University
Abstract
Background. Bitter melon (Momordica charantia L.) is a vegetable and medicinal crop new for Russia. Its high heat demand is the main factor limiting the possibilities of its cultivation in a temperate continental climate. Evaluation and selection of more cold-resistant accessions is required for breeding new cultivars and expanding production. Previously obtained data attest to a possibility of assessing cold hardiness in the mature male gametophyte phase in vitro.Materials and methods. Eight bitter melon accessions, including parent forms, F hybrids and cv. ‘Gosha’, were grown in the unheated film greenhouse of the Central Siberian Botanical Garden, Novosibirsk (54°49’ N, 83°06’ E) for evaluation and selection for cold resistance. The ratio of pollen germination in vitro on a 15% polyethylene glycol solution with a molecular weight of 6000 (PEG 6000) with the addition of boric acid (0.006%) at a temperature of 10°C for 24 h to the same indicator at 25°C / 3 h (in %) was used as a criterion of cold resistance.Results. The cultivar ‘Gosha’, the accession Okinawa green from Japan, and the F1 hybrid (Feng Guang × Okinawa green) had the highest cold resistance, with the values of 59.7; 53.8 and 48.2%, respectively. The inheritance of cold resistance in F , assessed by the pollen germination index, depending on the crossing combination, changed from intermediate in two hybrids [F1 Feng Guang × Gosha) and F1 Feng Guang × Okinawa green)] to overdominant (positive heterosis) in the hybrid F1 (Feng Guang × Okinawa white).
Publisher
FSBSI FRC N.I. Vavilov All-Russian Institute of Plant Genetic Resources
Subject
Plant Science,Genetics,Molecular Biology,Physiology,Biochemistry,Ecology, Evolution, Behavior and Systematics,Biotechnology
Reference33 articles.
1. Akbar S. Momordica charantia L. (Cucurbitaceae). In: Handbook of 200 Medicinal Plants. Cham: Springer; 2020. p.1195-1219. DOI: 10.1007/978-3-030-16807-0_128
2. Bagi I., Böszörményi A. Wild cucumber (Echinocystis lobata Torr. et Gray). In: Z. Botta-Dukát, L. Balogh (eds). The most important invasive plants in Hungary. Vácrátót; 2008. p.103-114.
3. Beaudry F.E.G., Rifkin J., Barrett S.C.H., Wright S.I. Evolutionary genomics of plant gametophytic selection. Plant Communications. 2020;1(6):100-115. DOI: 10.1016/j.xplc.2020.100115
4. Bitter gourd: [site]. Available from: https://kvk.icar.gov.in/API/Content/PPupload/k0211_74.pdf [accessed February 26, 2022].
5. Desai U.T., Musmade A.M. Pumpkins, squashes and gourds. In: D.K. Salunkhe, S.S. Kadam (eds). Handbook of vegetable science and technology: production, composition, storage and processing. New York: Marcel Dekker; 1998. p.273-298.