Effects of nanoselenium as a nutrient medium component on the main cultivation parameters and antagonistic activity of Lactobacillus strains

Author:

Omelchenko A. A.1,Rzhevskaya V. S.1,Kryzhko A. V.2,Panov D. A.1,Bugara I. A.1

Affiliation:

1. V.I. Vernadsky Crimean Federal University

2. Institute of Agriculture of Crimea

Abstract

The study aims to establish the effect of nanoselenium on the growth and antagonistic activity of the Lactobacillus casei IMB B-7343 and Lactobacillus plantarum IMB B-7344 strains against the phytopathogenic bacterium Xanthomonas campestris B-4102. Selenium nanoparticles were obtained by reducing a solution of sodium selenite with L-cysteine in the presence of sodium alginate. The cultivation of Lactobacilliwas carried out in a MRS nutrient medium with the addition of a colloidal solution of nanoselenium at the following concentrations: 0.05; 0.1; 0.15; 0.2 and 0.25 mg/l (for selenium). Bacteria were cultured in a 96-well plate in a Multiskan FC photometer at 36 ºС under constant shaking. The antagonistic activity of the strains was studied by the method of agar blocks. It was found that the addition of nanoselenium to the nutrient medium at a concentration of 0.2 to 0.25 mg/l contributed to a decrease in the duration of the exponential growth phase by an average of 3 hours, as well as to a decrease in the biomass accumulation of the L. casei IMB B-7343 strain by 15.0%. The optical density of this strain culture was observed to increase by 15.9% in the stationary growth phase under the addition of a nanoselenium solution to the nutrient medium at a concentration of 0.15 mg/l. During the cultivation of the L. plantarum IMB B-7344 strain in an MRS nutrient medium with the addition of a colloidal solution of nanoselenium at a concentration of 0.05–0.25 mg/l in the exponential development phase, an acceleration of the culture growth on average by 5 hours relative to the control was observed. It was noted that, upon the addition of nanoselenium to the nutrient medium, an increase in the antagonistic activity of lactobacillus strains against the phytopathogenic bacterium X. campestris B-4102 was observed, particularly in the L. plantarum IMB B-7344 strain (the zone of inhibition of the phytopathogen growth was 14.5–15 mm).

Publisher

Irkutsk National Research Technical University

Subject

General Medicine

Reference32 articles.

1. Sanin SS, Motovilin AA, Korneva LG, Zhokhova TP, Polyakova TM, Akimova EA. Chemical protection of wheat from the diseases under the conditions of intensive grain production. Zashchita i karantin rastenii. 2011;8;3–10. (In Russian)

2. Egorov N.P., Shafronov O.D., Egorov D.N., Suleimanov E.V. Development and experimental estimation of application efficiency of new nanotechnology fertilizers in crop production. Vestnik Nizhegorodskogo universiteta im. N.I. Lobachevskogo = Vestnik of Lobachevsky University of Nizhni Novgorod. 2008;6;94–99. (In Russian)

3. Papkina AV, Perfileva AI, Zhivetev MA, Borovskiy GB, Graskova IA, Lesnichaya MV, et al. Effect of selenium and arabinogalactan nano-composite on viability of the phytopathogen Clavibacter michiganensis subsp. Sepedonicus. Doklady Biological Sciences. 2015;461(1):89–91. https://doi.org/10.1134/S001249661501010X

4. Perfileva AI, Nozhkina OA, Graskova IA, Sidorov AV, Lesnichaya MV, Aleksandrova GP, et al. Synthesis of selenium and silver nanobiocomposites and their influence on phytopathogenic bacterium Clavibacter michiganensis subsp. Sepedonicus. Russian Chemical Bulletin. 2018;67(1):157–163. https://doi.org/10.1007/s11172-018-2052-4

5. Perfileva AI, Nozhkina OA, Graskova IA, Dyakova AV, Pavlova AG, Klimenkov IV, et al. Selenium nanocomposites having polysaccharid matrices stimulate growth of potato plants in vitro infected with ring rot pathogen. Doklady Biological Sciences. 2019;489(1):184–188. https://doi.org/10.1134/S0012496619060073

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3