Effect of protatranes on the biosynthesis of extracellular enzymes of <i>Candida ethanolica</i> BKM Y-2300 T

Abstract

The study aims to explore the possibility of using synthetic biologically active compounds (protatranes) to increase the lipolytic and proteolytic activity of Candida ethanolica. Protatrane 1 (tris(2-hydroxyethyl) ammonium-4chlorophenyl-sulfanyl acetate) and protatrane 2 (tris(2-hydroxyethyl) ammonium-4-chlorophenyl-sulfonyl acetate) were added to the growth medium at trace concentrations of 1×10−6–1×10−8wt% separately and together. It was established that with the introduction to the growth medium, protatranes had a multidirectional effect on the biosynthesis of enzymes by Candida ethanolica yeast. This effect was dependent on the concentrations of these compounds, as well as on their separate or combined introduction. All the studied concentrations were found to be effective for lipase synthesis, as they improved enzyme synthesis by 1.7–8.6 times. The combined use of protatranes increased enzyme synthesis by 3.4–11.7 times. For protease formation, the combined introduction of the studied protatranes at a concentration of 10−6wt% was found to be the most effective, with enzyme synthesis reaching 184.8±7.02 U/mL in the culture broth. When co-introduced, the studied protatranes tris(2-hydroxyethyl) ammonium-4-chlorophenylsulfanyl acetate and tris(2-hydroxyethyl) ammonium-4-chlorophenyl-sulfonyl acetate can be used to increase the production efficiency of extracellular lipases and proteases by Candida ethanolica. The specific effect of protatranes was revealed to be the uneven dynamics of extracellular enzyme accumulation, manifested in the presence of sharp peaks during yeast cultivation. The reasons for this unevenness require further research.