Affiliation:
1. Siberian Institute of Plant Physiology and Biochemistry SB RAS
Abstract
The study set out to investigate the effect of different concentrations of copper ions on the composition of phytosterols of the vacuolar membrane (tonoplast) of beetroot (Beta vulgaris L.). To characterise the stress caused by the action of copper ions, a conductometric study of the permeability of cell membranes and the level of lipid peroxidation was carried out. The results demonstrate an increase in these indicators in beetroot tissues. Among tonoplast phytosterols, β-sitosterol, stigmasterol, campesterol, and cholesterol were studied. In cell membranes, these phytosterols perform a structural function, contributing to lipid microdomain formation, influencing plant growth and development, and participating in plant responses to stress. The study of the effect of copper ions on tonoplast phytosterols showed most of them to be occupied by the free form. In the presence of 100 μM copper, a significant increase in campesterol content was detected. The ratio of stigmasterol/β-sitosterol and 24-methyl-/ ethylsterol, which may influence the stress response of plants, is an important indicator of plant membrane health. The observed increases in the ratios of stigmasterol/β-sitosterol and 24-methyl-/ethylsterols can be explained in terms of mechanisms for regulating the functioning of the tonoplast under stress caused by copper ions. Thus, the obtained results may indicate the participation of cell membranes, including tonoplast, in the mechanisms of adaptation of beetroot tissue cells to stress caused by copper ions.
Publisher
Irkutsk National Research Technical University
Reference31 articles.
1. Rahman Z., Singh V.P. The relative impact of toxic heavy metals (THMs) (arsenic (As), cadmium (Cd), chromium (Cr) (VI), mercury (Hg), and lead (Pb)) on the total environment: an overview. Environmental Monitoring and Assessment. 2019;191:419. DOI: 10.1007/s10661-019-7528-7.
2. Feki K., Tounsi S., Mrabet M., Mhadhbi H., Brini F. Recent advances in physiological and molecular mechanisms of heavy metal accumulation in plants. Environmental Science and Pollution Research. 2021;28:64967-64986. DOI: 10.1007/s11356-021-16805-y.
3. Jiang Y.-T., Tang R.-J., Zhang Y.-J., Xue H.-W., Ferjani A., Luan S., et al. Two tonoplast proton pumps function in Arabidopsis embryo development. New Phytologist. 2020;225(4):1606-1617. DOI: 10.1111/nph.16231.
4. Los’ D.A. Perception of stress signals by biological membranes. In: Rubin A.B. (ed.). Problemy regulyatsii v biologicheskikh sistemakh. Biofizicheskie aspekty = Problems of regulation in biological systems. Biophysical aspects. Moscow – Izhevsk: Scientific Center “Regulatory and chaotic dynamics”, Institute for Computer Research; 2007, p. 329-360. (In Russian).
5. Valitova J.N., Sulkarnayeva A.G., Minibayeva F.V. Plant sterols: diversity, biosynthesis, and physiological functions. Biokhimiya. 2016;81(8):1050-1068. (In Russian). EDN: WJXJID.
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献