Abstract
Sensitive and accurate spectrophotometric method for the assay of methyldopa (MeD) as pure form and in it is formulations. The method was centred on the oxidation of methyldopa drug with Fe+2ions. The free Fe+2 ions react with 4,7-diphenyl-1,10-phenanthroline chromogenic reagent. In the method, the formed Ferron complex is colorimetrically can be monitored at 533 nm versus reagent blank. The linear relationship between various MeD concentrations versus absorbance for each concentration was in the range of 0.25-2.5 μg.mL-1 with molar absorptivity 1.3272x105 L.mol-1.cm-1 for the method. The determination coefficient (R2) was found to be 0.9902, and the limit of detection (LOD) and limit of quantification(LOQ) were 0.011μg.ml-1and 0.036 μg.ml-1, respectively. The percent recovery from 99.84% to 102.40 %. The suggested procedure could be used for the estimation of MeD in dosage forms (two types of tablets from two companies) with satisfactory results.
Publisher
International Research and Publishing Academy
Reference20 articles.
1. Sweetman SC .2007 “Martindale: The Complete Drug R” .35th Ed. pharmaceutical press. London, UK
2. British Pharmacopoeia on CD-ROM 3.0, a component ,BP2007.
3. Bong K.Kim ,Robert T. Koda , Fluorometric Determination of Methyldopa in Biological Fluids, Journal of Pharmaceutical Sciences , volume 66,p:1632-1634, 1977.- December 2016; Article No. 46, Pages: 247-251.
4. Suzan yanik & Saliha B. Kurt & Betul Ari & Sahin Demirci& Selehattin Yilmaz, Development of Electrochemical Sensors for Quantitative Analysis of Methyldopa at Modified-gce and pge Electrodes by Voltammetry , Journal of Scientific Perspectives volume 4, Issue 4, Year 2020, pp. 223-236.
5. M.V.L. Sahithi,, V.Perumal1,Chandra K Sekhar, P. VijayBabu ,Development and Validation of RP-HPLC method for simultaneous estimation of Methyldopa and Hydrochlorothiazide in Pharmaceutical Dosage Form, ournal of Advanced Pharmacy Education & Research, 3(4):464-470,2019.