Different Laboratory Techniques of Streptococcus Pyogenes Isolated from Inflammatory Secretions of Skin

Abstract

Background: Streptococcus pyogenes is a well-known human pathogen that causes a variety of illnesses, from simple skin infections to more serious invasive problems. The prevention of sequelae such as rheumatic fever and post-streptococcal glomerulonephritis is dependent on an accurate diagnosis. Objective: Our main objective is to thoroughly study S. pyogenes in pus-leaking skin infections, by using methods like PCR amplification. These advanced techniques will help us to gain new insights into the prevalence and significance of S. pyogenes in this particular infection, thereby enhancing our current understanding of the subject. Methods: We studied sixty grown-up patients with exudative skin problems. We collected samples by swabbing fluid from their inflamed areas and putting it on special plates. Then we used the boiling method to isolate the DNA and amplify the Spy 1258 gene with PCR. Results: Thirty-six (or sixty percent) of the sixty samples tested under culture were S. pyogenes positive. S. pyogenes' presence in these samples was further evidenced by the production of a unique DNA fragment with a length of 407 base pairs using PCR amplification. Conclusion: Apart from underlining the need of using reliable diagnostic instruments, the study reveals that S. pyogenes is rather common in exudative skin disorders. Correct antibiotic treatment resulting from precision and timely diagnosis is essential to avoid major adverse effects. Treatment approaches will be improved by further investigation on the genetic variety of S. pyogenes and its antibiotic resistance.