The effect of the gibberellic acid on the morphogenesis of Hydrangea L.

Abstract

Modern ornamental gardening is difficult to imagine without beautifully flowering shrubs. One of these is hydrangea. In Russia, culture is one of the most popular in decorative gardening. However, there are some difficulties in obtaining a large amount of planting material of new and highly ornamental varieties. Therefore, it is important to develop effective reproduction technologies of the studied culture. It is possible to obtain high-quality planting material by clonal micropropagation in a short period of time. The resulting plants grow similar to the mother plant. Hydrangea L. representatives are effectively cultivated in vitro. There are many results of research about micropropagation of hydrangea, but there is only isolated datas about the comparative assessment of the morphogenetic potential of various species of the Hydrangea L. depending on the concentration of growth regulators. There are many growth regulators in the practice of cultivation in vitro. 6-benzylaminopurine (6-BAP), gibberellic acid, kinetin, zeatin. Murashige (MS) and Skoog and Quoirin (QL), Lepoivre nutrient media are used to activate the growth of axillary buds at the stage of micro-reproduction proper. The effect of the use of gibberellins on the in vitro cultivation of hydrangeas is poorly studied. The research aimed to studing the effect of gibberellic acid on the regeneration of shoots of Hydrangea L. We study the effect of gibberellic acid (GA) – 0.5 and 1 mg/l of concentrations. Reproduction coefficient of the Hydrangea macrophylla Thunb. (H. macrophylla) was 12.0, when we used 1 mg / l GA. The length of the Hydrangea paniculata Siebold (H. paniculata) micro-shoots increased by 25 % when we used 1 mg/l GA. According to the research of 2020, the best results on the use of different nutrient mediums mineral bases were obtained during in vitro cultivation on a QL nutrient medium with the addition of 0.3 mg/l 6-BAP. In the future this nutrient medium was as a control. After the application of GA, regenerants were planted to adapt of phytotron conditions. The best survival rate of H. macrophylla (90 %) (var. Peppermint) was recorded after cultivation of a nutrient medium containing 1 mg/l GA. The adaptability of ‘Magical Candle’ was excellent regardless of GA concentration.