Increased Synthesis and AVP Unresponsiveness of Na,K-ATPase in Collecting Duct from Nephrotic Rats

Author:

DESCHÊNES GEORGES,GONIN SANDRINE,ZOLTY EINATH,CHEVAL LYDIE,ROUSSELOT MARTINE,MARTIN PIERRE-YVES,VERBAVATZ JEAN-MARC,FÉRAILLE ERIC,DOUCET ALAIN

Abstract

Abstract. Renal sodium retention is responsible for ascites and edema in nephrotic syndrome. In puromycin aminonucleoside (PAN)—induced nephrosis, sodium retention originates in part from the collecting duct, and it is associated with increased Na,K-ATPase activity in the cortical collecting duct (CCD). The aims of this study were to evaluate whether the outer medullary collecting duct (OMCD) also participates to sodium retention and to determine the mechanisms responsible for stimulation of Na,K-ATPase in CCD. PAN nephrosis increased Na,K-ATPase activity in the CCD but not in OMCD. The two-fold increase of Na,K-ATPase activity in CCD was associated with two-fold increases in the number of α and β Na,K-ATPase subunits mRNA determined by quantitative RT-PCR and of the total amount of Na,K-ATPase α subunits estimated by Western blotting. PAN nephrosis also increased two-fold the amount of Na,K-ATPase α subunit at the basolateral membrane of CCD principal cells, as determined by Western blotting after biotinylation and streptavidin precipitation and by immunofluorescence. The intracellular pool of latent Na,K-ATPase units also increased in size and was no longer recruitable by vasopressin and cAMP. This unresponsiveness of the intracellular pool of Na,K-ATPase to vasopressin was not the result of any alteration of the molecular and functional expression of the vasopressin V2receptor/adenylyl cyclase (AC) complex. It is concluded that PAN nephrosis (1) does not alter sodium reabsorption in OMCD, (2) is associated with increased synthesis and membrane expression of Na,K-ATPase in the CCD, and (3) alters the normal trafficking of intracellular Na,K-ATPase units to the basolateral membrane.

Publisher

American Society of Nephrology (ASN)

Subject

Nephrology,General Medicine

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