Çardak Lagünü'nde (Çanakkale Boğazı) bulunan portunid yengeç, Carcinus aestuarii Nardo, 1847'nin hemolenfinde çevresel değişkenlerin ve morfometrinin hemosit yapısına etkileri

Author:

ACAR Seçil1,ATEŞ A. Suat2

Affiliation:

1. ÇANAKKALE ONSEKİZ MART ÜNİVERSİTESİ, DENİZ BİLİMLERİ VE TEKNOLOJİSİ FAKÜLTESİ

2. ÇANAKKALE ONSEKİZ MART ÜNİVERSİTESİ

Abstract

The aim of this study is to determine the relationship between hemocyte count and hemocyte types with environmental variables and morphometric measurements in Carcinus aestuarii. A total of 240 crabs were seasonally collected in Çardak Lagoon between June 2018 and May 2019. Crab specimens were anesthetized on ice for 10 min, and 500 µL of hemolymph was collected from the legs of each crab using a 1-mL plastic syringe. After sampling, hemolymph samples was mixed with the anticoagulant, it was applied to a Thoma slide and examined under a microscope at X40 magnification. The total hemocyte count (THC) was calculated as 13.91±8.08 x106. Differential hemocyte count (DHC) 53.25±0.6 for hyalinocyte, 26.15±0.23 for semi-granulocyte and 20.6±0.06 for granulocyte and 3 different cell types were observed. The most dominant cell type is hyalinocyte cells.

Publisher

Suleyman Demirel Universitesi Egirdir Su Urunleri Fakultesi Dergisi

Subject

Hardware and Architecture,Geology,Geotechnical Engineering and Engineering Geology

Reference21 articles.

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2. Aliko, V., Hajdaraj, G., Caci, A., & Faggio, C. (2015). Copper induced lysosomal membrane destabilisation in haemolymph cells of Mediterranean green crab (Carcinus aestuarii, Nardo, 1847) from the Narta Lagoon (Albania). Brazilian Archives of Biology and Technology, 58, 750-756. https://doi.org/10.1590/S1516-89132015050244

3. Bachau, A. G. (1981). Crustaeceans. Invertebrate blood cells, 2, 385-420.

4. Başusta A, Şen D. (2004). Keban Baraj Gölü’nde yaşayan Acanthobrama marmid (Heckel,1843) de kan parametrelerinin incelenmesi. Turkish Journal of Veterinary and Animal Sciences. 28, 1, 1-6.

5. Battison, A., Cawthorn, R., & Horney, B. (2003). Classification of Homarus americanus hemocytes and the use of differential hemocyte counts in lobsters infected with Aerococcus viridans var. homari (Gaffkemia). Journal of Invertebrate Pathology, 84(3), 177-197. https://doi.org/10.1016/j.jip.2003.11.005

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