GLN27GLU POLYMORPHISM IN THE Β2-ADRENORECEPTOR GENE IN PATIENTS WITH ASTHMA WITH REGARD TO THE AGE OF ONSET

Abstract

Aim: The study aimed to assess the frequency of the Gln27Glu polymorphic variant in the β2-AR gene among patients with early and late-onset asthma and assess asthma risks depending on the disease phenotype. Materials and Methods: Our study included a total of 553 asthma patients who consented to participate in the study. Asthma was diagnosed according to the 2016 GINA recommendations and its later versions. The study was approved by the Bioethics Committee of the Medical Institute of Sumy State University. The analysis for determining genetic polymorphism (designated as rs1042714) was conducted through the use of polymerase chain reaction-restriction fragment length polymorphism. Statistical analysis of obtained results was performed using SPSS–17 program. Results: It was found that there is a significant difference in the distribution of alleles and genotypes in people with early-onset asthma compared to those with late-onset asthma; the statistical analysis showed a χ2 value of 41.75 and p-value of 0.001 for early-onset asthma, and a χ2 value of 44.24 and p-value of 0.001 for late-onset asthma. We did not observe a significant increase in the early-onset asthma risk with an account of different inheritance models connected to the studied polymorphism. Research that took into account the risk of late-onset asthma discovered statistically significant results regarding the dominant (p = 0.001), super-dominant (p = 0.001), and additive (p = 0.001) models of inheritance. Conclusions: Based on the data collected, it was found that individuals carrying the minor allele (both homozygotes and heterozygotes) were at a greater risk of developing asthma later in life. However, no such correlation was observed in patients with early-onset asthma.