Affiliation:
1. EGE ÜNİVERSİTESİ, EGE TIP FAKÜLTESİ
2. HARRAN ÜNİVERSİTESİ, TIP FAKÜLTESİ
Abstract
Introduction: Hypertension is a systemic disease characterized by high blood pressure and an important threat for the population, as it is common and can cause serious complications. Genetic and environmental factors are involved in its development like the recently defined genetic risk factor ARHGAP42 that encodes the Rho GTPase activating protein 42. In the study, the intronic rs604723 (C/T) gene polymorphism of ARHGAP42 was investigated in relation to arterial stiffness.
Methods: Peripheral blood samples, taken from 63 study group subjects with hypertension and 100 healthy subject as control, were analyzed for the presence of the ARHGAP42 rs604723 gene polymorphism by a real-time PCR method following DNA isolation. Demographic data of the study group subjects were recorded and blood pressure, ambulatory blood pressure and arterial stiffness values were measured.
Results: The heterozygous polymorphic CT (~2-fold) and homozygous polymorphic TT (~1.6-fold) genotypes were found to be higher in study group subjects when compared to the control group subjects, whereby the increase of the CT genotype was statistically significant (p = 0.006). Similarly, the frequency of the polymorphic T allele was found to be higher (~1.9-fold) and statistically significant (p = 0.003) in the study group subjects. In addition, the heterozygous polymorphic CT and homozygous polymorphic TT genotypes were found to be associated with carotid-femoral pulse velocity, which is a measure of arterial stiffness (p = 0.025).
Conclusion: In this study, the ARHGAP42 rs604723 (C/T) gene polymorphism was found to be associated with pulse wave speed in subjects with hypertension. It will be of interest, to investigate its association with any specific drug or drugs commonly used in anti-hypertensive therapy. Thus, it would be possible to select the appropriate drug or drugs according to the hypertensive subjects’ genotype to carry out personalized medicine in future.
Funder
This study evaluated by the Medical Health Group was approved as project ID 20208 and funded by the Ege University Research Fund.
Publisher
Harran Universitesi Tip Fakultesi Dergisi
Reference24 articles.
1. 1. Li C, He J, Chen J, Zhao J, Gu D, Hixson JE, et al. Genome-wide gene–sodium interaction analyses on blood pressure: the genetic epidemiology network of salt-sensitivity study. Hypertension. 2016;68(2):348-55.
2. 2. Bai X, Lenhart KC, Bird KE, Suen AA, Rojas M, Kakoki M, et al. The smooth muscle-selective RhoGAP GRAF3 is a criti-cal regulator of vascular tone and hypertension. Nature communications. 2013; 13:4(1):2910.
3. 3. Bai X, Mangum K, Kakoki M, Smithies O, Mack CP, Taylor JM. GRAF3 serves as a blood volume-sensitive rheostat to control smooth muscle contractility and blood pressure. Small GTPases. 2020; 11(3): p. 194-203.
4. 4. Bai X, Mangum KD, Dee RA, Stouffer GA, Lee CR, Oni-Orisan A, et al. Blood pressure–associated polymorphism controls ARHGAP42 expression via serum response factor DNA binding. The Journal of clinical investigation. 2017;127(2):670-80.
5. 5. Oh YS. Arterial stiffness and hypertension. Clin Hypertens. 2018; 24: p.17.