Hemostatic Efficacy of Algan Hemostatic Agent in Renal Vein Incision Model in Rats

Abstract

Background: The aim of this study is to evaluate the acute hemostatic effects of liquid and powder forms of AHA in severe bleeding model of rat renal vein. Materials and Methods: 10-12 weeks old, 24 male Wistar albino rats were randomly divided into three groups of eight animals each as the control, AHA liquid, AHA powder. The left renal artery and vein were revealed with a 3 cm vertical incision and the tip of the injector was used to puncture renal vein, causing bleeding. In all animals, regular gauze patch was pressed over the incised area for 10 seconds as soon as the bleeding began. Immediately after removing the sponge; physiological saline, AHA liquid and powder were applied with compress to injured site of relevant groups for 2 minutes, respectively. The time was recorded by chronometer and the area was checked after two minutes. Hemostasis that was not achieved after three applications was accepted as failure. Results: Cessation of the bleeding failed in all rats after three successive 2-minute physiological saline impregnated gauze applications in control group. Among AHA-treated groups, significant hemostasis efficacy was obtained from the liquid form than that of powder. Within first two minutes, bleeding in renal veins of five rats was controlled successfully in liquid group whereas powder group achieved hemostatic success in four rats. Conclusions: The vessel incision model in our study revealed fast-acting physical hemostatic properties of the liquid and powder formulations of AHA in the renal vein. This natural applicable product could be used to induce hemostasis in rat models of bleeding caused by various renal damages or trauma, and it could also prevent local bleeding in humans. More studies are needed to compare the efficacy of different formulations of the polysaccharide-based herbal product in various organs and surgical models.