ASSOCIATION OF THE TLR9 GENE rs352140 POLYMORPHISM WITH THE ORAL MUCOSA HYPERKERATOSIS DEVELOPMENT

Abstract

Oral diseases affect about 3.5 billion people. One of these diseases is a disorder of keratinization of the oral mucosa: parakeratosis, dyskeratosis, keratosis, hyperkeratosis, acanthosis. It was of interest to us that the increased risk of cancer is statistically associated with the TT genotype of the TLR9 polymorphism (rs352140. It is also known to play a significant role in carcinogenesis through diffuse TLR2 overexpression, even in early lesions before the onset of dysplasia. There is practically no data on the association of TLR9 gene polymorphism with the risk of developing a violation of the process of keratinization of the oral mucosa. Objectives. To identify the association between the carrier of TLR9 rs352140 polymorphism and the likelihood of hyperkeratosis. Methodology. DNA extraction for the study of single-nucleotide polymorphic markers was carried out from buccal epithelial cells using a commercial kit "RIBO-sorb", reagents from the “Set of reagents for RT-PCR” (Syntol, RF) and specially synthesized primers and probes (Syntol, RF) were used. The study involved 60 patients, 30 of whom had a confirmed hyperkeratosis of the oral mucosal, and other 30 were in the comparison group. Samples diagnosed with hyperkeratosis were selected based on single lesions with or without dysplasia and included all areas of the oral mucosa. Results. The number of patients with oral hyperkeratosis who are carriers of the TT genotype allele of the TLR9 rs352140 gene was 50.0% (the criterion χ2 it was 6.50 (p < 0.05), and the Fisher criterion was p < 0.05). Conclusion. The association between the TLR9 rs352140 polymorphism (TT genotype) and the development of hyperkeratosis of the oral cavity: the TT genotype and the T allele of the single nucleotide polymorphic marker rs352140 are predictors of the risk of developing hyperkeratosis of the oral mucosal.